BANDAAQUA: Jurnal Budidaya Perairan Universitas Banda Naira

DETECTION OF MEGALOCYTIVIRUS IN CLOWNFISH (Amphiprion ocellaris)

2026-09-15T04:04:08+00:00

This study aimed to detect the presence of Megalocytivirus in clownfish (Amphiprion ocellaris) obtained from the Ambon Marine Aquaculture Center (BPBL). The research used a descriptive laboratory, non-experimental approach, with molecular detection performed through Polymerase Chain Reaction (PCR) and nested PCR techniques. Five clownfish specimens (approximately 3 cm) were sampled, and spleen tissue was used as the target organ for analysis. The extracted DNA was assessed for concentration and purity before amplification by PCR and nested PCR. The amplified products were analyzed using agarose gel electrophoresis to identify the presence of viral DNA bands. The results showed no Megalocytivirus-specific DNA bands in any of the samples, whereas the positive control produced the expected result, confirming the validity of the testing procedure. The study concluded that all clownfish samples from BPBL Ambon were negative for Megalocytivirus infection and were considered free from the virus at the time of sampling.

BUSINESS ANALYSIS OF GIANT TREVALLY (Caranx sp.) CULTIVATION USING A FLOATING NET CAGE SYSTEM IN THE WATERS OF LAUTAKA HAMLET, BANDA NEIRA, CENTRAL MALUKU

2026-10-27T01:58:41+00:00

This study aimed to analyze the technical performance and economic feasibility of giant trevally (Caranx sp.) cultivation using a floating net cage (FNC) system in the waters of Lautaka Hamlet, Banda Neira, Central Maluku. The study was conducted for two months, from November to December 2025, using observation, interviews, documentation, and literature review to collect technical and economic data. The data were analyzed descriptively. The cultivation system used three traditional floating net cage compartments, each measuring 3 × 2 × 2 m, with a stocking density of 250 fish per compartment. The fish were fed trash fish at approximately 40 kg day⁻¹. The survival rate was 83%, feed conversion ratio (FCR) was 1.2, and specific growth rate (SGR) was 0.59% day⁻¹. Total production cost was Rp 18,540,000, while total revenue reached Rp 25,800,000, resulting in a profit of Rp 7,260,000 per production cycle. The reported Return Cost Ratio (RCR) was 1.3, indicating that the enterprise was economically feasible. The reported payback period was 2.5 years, while the break-even point (BEP) was 309 fish. Overall, giant trevally cultivation using the FNC system in Lautaka Hamlet has potential for development as a sustainable source of income for coastal communities, provided that technical and financial management is improved.

CALCIUM AND PHOSPHATE CONCENTRATIONS IN COASTAL WATERS AT THE PARIGI DUA FLOATING NET CAGE AQUACULTURE SITE, BANDA NAIRA, CENTRAL MALUKU, INDONESIA

2026-10-27T05:05:49+00:00

This study assessed calcium (Ca) and phosphate concentrations in seawater at the Parigi Dua floating net cage (FNC) aquaculture site in Nusantara Village, Banda Naira, Central Maluku, Indonesia. Seawater samples were collected at a depth of 8 m during August–September 2025. Calcium and phosphate were measured using Hanna HI758 Calcium Checker and Hanna HI713 Phosphate Checker instruments, respectively, while supporting water-quality parameters were measured in situ. The mean water temperature was 28.3 °C, salinity 30 ppt, pH 7.78, and dissolved oxygen (DO) 5.3 mg L⁻¹. Calcium concentrations were reported to range from 418 to 600 mg L⁻¹, with a mean of 509 mg L⁻¹. Phosphate concentrations were 0.11 mg L⁻¹ in August and 0.90 mg L⁻¹ in September, giving a reported mean of 0.505 mg L⁻¹. This mean is substantially higher than the 0.015 mg L⁻¹ phosphate criterion cited by the original study from Indonesian Government Regulation No. 22/2021. The findings identify phosphate enrichment as a priority for follow-up monitoring around the aquaculture site. However, the descriptive design and limited temporal sampling do not establish the source of phosphate or demonstrate that aquaculture was its cause. Further work should include replicated sampling across stations, depths, tides, and seasons, alongside quality assurance and source assessment.